RNA bridges the gap between DNA and proteins, offering a real-time view into how an individual’s body responds to disease and treatment. Unlike DNA, which remains relatively constant, RNA expression levels fluctuate in response to physiological changes, making them excellent biomarkers for disease progression and therapeutic outcomes.

While proteins (such as troponin, ALT, and PSA) have been traditionally used as biomarkers, current proteomics technologies can only detect a fraction of the entire protein complement. In contrast, RNA-sequencing (RNA-seq) offers comprehensive profiling across more than 27,000 genes from a single sample, enabling broader and more detailed analyses.

Advances in high-throughput sequencing technologies over the past 15 years have revolutionised RNA expression profiling, from early RT-qPCR and microarrays to modern, expansive RNA-seq tests. This evolution allows for dynamic and detailed insights into cellular activity, with applications spanning diagnostics, treatment monitoring, and drug development.

By prioritising RNA analysis, Bayosthiti AI captures the most responsive and informative aspects of an individual’s biology. Although a complete biostate or bayosthiti requires data from multiple molecular types, RNA remains the most advanced and reliable indicator of real-time health status. It’s akin to scanning the headlines of a newspaper: while you might miss some fine print, the overall story is clear and compelling. Moreover, with sufficiently large datasets and sophisticated AI, even subtle RNA-based signals can be leveraged for powerful diagnostic and therapeutic predictions.

Despite its power, RNA-seq adoption in routine diagnostics faces hurdles such as high per-sample costs, complex workflows, and scalability issues. Bayosthiti’s breakthrough technologies—BIRT (Barcode-Integrated Reverse Transcription) and PERD (Probes for Excess RNA Depletion)—address these challenges by streamlining workflows and reducing costs by 2-3x, making high-quality RNA analysis more accessible and efficient.